How can I buy methylated Adenine?

I have used 5mC (5' methyl cytosine) to impair Acc65I recognition sites randomly in linearized repetitive sequences, but I want to build up with methylated adenine nomoners for PCR amplification....

31 December 2018 9,106 0 View

Does anyone know why my E. coli growth media waste has dark-brown color (or black)?

31 December 2016 7,667 3 View

Why does my e coli colony on a plate behave strangely?

All- I am running an experiment for protein expression with e. coli and my LB plate behave strangely these days. As you see in the attached picture, even in several days after transformation,...

10 November 2016 6,724 4 View

Does anyone know what can happen if my bacteria culture does not have enough Kanamycin while protein production?

HI all, I am wondering how Kanamycin works on protein production in e. coli. I heard that Kanamycin and Ampicillin have different mechanism for controlling cell growth.I learned how Amlicillin...

05 June 2016 755 2 View

Does anyone know the rough calculation on the linear length (in nm or A) of polypeptide chain?

03 April 2016 2,889 8 View

Can I remove urea and triton X-100 with dialysis?

Hi, I am working on recombinant protein production and I want to know whether I can remove urea and triton-X100 with dialysis. After the lysis of my cell pellet, I usually use 2M urea with...

01 February 2016 6,508 3 View

I am looking for 350kDa protein with precast SDS gel. Can I use the gel when my protein is not in the separation range?

Hi, I am using precast gel (Bolt 4-12% Bis-Tris precast gel) and in its specification the separation range of the gel is 3.5kDa - 150kDa. I am running some protein samples with size of 300 - 400...

31 December 2015 4,963 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

Does anyone have a sequence for the human CD34 promoter?

I'm looking for the human CD34 promoter sequence to engineer into a vector construct. Can anyone recommend a good source for this sequence? Thanks very much, Laura

25 February 2021 8,205 1 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View