Does anyone know what is the role of BOM (basis of mobility) in pET28b?

01 February 2017 1,620 1 View

Does anyone know why my E. coli growth media waste has dark-brown color (or black)?

31 December 2016 7,667 3 View

Why does my e coli colony on a plate behave strangely?

All- I am running an experiment for protein expression with e. coli and my LB plate behave strangely these days. As you see in the attached picture, even in several days after transformation,...

10 November 2016 6,724 4 View

Does anyone know what can happen if my bacteria culture does not have enough Kanamycin while protein production?

HI all, I am wondering how Kanamycin works on protein production in e. coli. I heard that Kanamycin and Ampicillin have different mechanism for controlling cell growth.I learned how Amlicillin...

05 June 2016 755 2 View

Does anyone know the rough calculation on the linear length (in nm or A) of polypeptide chain?

03 April 2016 2,889 8 View

Can I remove urea and triton X-100 with dialysis?

Hi, I am working on recombinant protein production and I want to know whether I can remove urea and triton-X100 with dialysis. After the lysis of my cell pellet, I usually use 2M urea with...

01 February 2016 6,508 3 View

I am looking for 350kDa protein with precast SDS gel. Can I use the gel when my protein is not in the separation range?

Hi, I am using precast gel (Bolt 4-12% Bis-Tris precast gel) and in its specification the separation range of the gel is 3.5kDa - 150kDa. I am running some protein samples with size of 300 - 400...

31 December 2015 4,963 4 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

Which DL architecture is better to work on handwritten data?

I am working in the area of Handwriting recognition for Bangla. As there are many DL architecture, I am confused which one to select.

15 February 2021 4,996 1 View

How to obtain regions around the person bounding boxes(excluded the detected people in scene)?

Hi all. I have a set of coordinates of bounding boxes for AVA Dataset. This dataset is used in action recognition. My goal is to get the areas around the people (people are inside boxes) and do...

13 February 2021 2,538 2 View

Can anyone pls provide me information regarding the libraries where from i can get the absorption data of different materials used in solar cells??

I want to create .abs file ( absorption) for simulating solar cell in SCAPS. Can anyone pls provide me information regarding the libraries (websites) where from i can get the absorption data of...

10 February 2021 6,034 7 View