How can I buy methylated Adenine?

I have used 5mC (5' methyl cytosine) to impair Acc65I recognition sites randomly in linearized repetitive sequences, but I want to build up with methylated adenine nomoners for PCR amplification....

31 December 2018 9,106 0 View

Does anyone know what is the role of BOM (basis of mobility) in pET28b?

01 February 2017 1,620 1 View

Does anyone know why my E. coli growth media waste has dark-brown color (or black)?

31 December 2016 7,667 3 View

Why does my e coli colony on a plate behave strangely?

All- I am running an experiment for protein expression with e. coli and my LB plate behave strangely these days. As you see in the attached picture, even in several days after transformation,...

10 November 2016 6,724 4 View

Does anyone know what can happen if my bacteria culture does not have enough Kanamycin while protein production?

HI all, I am wondering how Kanamycin works on protein production in e. coli. I heard that Kanamycin and Ampicillin have different mechanism for controlling cell growth.I learned how Amlicillin...

05 June 2016 755 2 View

Can I remove urea and triton X-100 with dialysis?

Hi, I am working on recombinant protein production and I want to know whether I can remove urea and triton-X100 with dialysis. After the lysis of my cell pellet, I usually use 2M urea with...

01 February 2016 6,508 3 View

I am looking for 350kDa protein with precast SDS gel. Can I use the gel when my protein is not in the separation range?

Hi, I am using precast gel (Bolt 4-12% Bis-Tris precast gel) and in its specification the separation range of the gel is 3.5kDa - 150kDa. I am running some protein samples with size of 300 - 400...

31 December 2015 4,963 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View