Huihun Jung

8 Questions 4 Answers 0 Followers

Questions related from Huihun Jung

How can I buy methylated Adenine?

I have used 5mC (5' methyl cytosine) to impair Acc65I recognition sites randomly in linearized repetitive sequences, but I want to build up with methylated adenine nomoners for PCR amplification....

01 January 2019 9,139 0 View

Does anyone know what is the role of BOM (basis of mobility) in pET28b?

Hello all- I am working with protein expression with a derivative of pET28b vector and I found there are three nucleotides deletion in BOM sites in my vector. Does anyone know what is the role of...

02 February 2017 1,658 1 View

Does anyone know why my E. coli growth media waste has dark-brown color (or black)?

Hi all- I have been conducted my research for protein expression with e. coli (BL21-DE3 strain) and I usually use BLEACH for my liquid waste (usually LB waste). And what I found is the color of...

01 January 2017 7,696 3 View

Why does my e coli colony on a plate behave strangely?

All- I am running an experiment for protein expression with e. coli and my LB plate behave strangely these days. As you see in the attached picture, even in several days after transformation,...

11 November 2016 6,758 4 View

Does anyone know what can happen if my bacteria culture does not have enough Kanamycin while protein production?

HI all, I am wondering how Kanamycin works on protein production in e. coli. I heard that Kanamycin and Ampicillin have different mechanism for controlling cell growth.I learned how Amlicillin...

06 June 2016 789 2 View

Does anyone know the rough calculation on the linear length (in nm or A) of polypeptide chain?

I am wondering about the rough calculation for linear polypeptide chain length in nanometer. For example, if I have a linear chain of 10 amino acid polypeptide, then what nano meter length they...

04 April 2016 2,920 8 View

Can I remove urea and triton X-100 with dialysis?

Hi, I am working on recombinant protein production and I want to know whether I can remove urea and triton-X100 with dialysis. After the lysis of my cell pellet, I usually use 2M urea with...

02 February 2016 6,571 3 View

I am looking for 350kDa protein with precast SDS gel. Can I use the gel when my protein is not in the separation range?

Hi, I am using precast gel (Bolt 4-12% Bis-Tris precast gel) and in its specification the separation range of the gel is 3.5kDa - 150kDa. I am running some protein samples with size of 300 - 400...

01 January 2016 4,994 4 View