Can I use carbo-free blocking solution in place of normal goat serum?

I'm looking to try this in place of normal goat serum for a wisteria floribunda lectin stain. In my IF protocol, 10% of the final volume of my blocking solution is goat serum. If I were to go the...

07 February 2021 7,520 6 View

How do I delete a publication that I added?

I added two times the same thing by mistake and I need to remove one. Thanks.

11 January 2021 6,118 5 View

PScad compatibility with SIMULINK?

Hello, I tried to perform a co-simulation between PScad and SIMULINK. I reviewed the requirements of compatibility between them but I still have no clue about this issue. My PScad is 4.6.3 (x64),...

06 January 2021 8,226 1 View

Journals with low APC or no APC?

Do you know any journals in the area of medicine/pediatrics/nutrition that publish protocols of systematic reviews? No APC or Low APC

04 January 2021 292 14 View

Any suggestion for counting a large amount of small seeds?

I am currently working with S. oleraceus and I would like to know if there is a practical method to accurately counting the seeds other than weighing them or simply counting one by one? Thank you...

02 December 2020 6,988 3 View

Can problems with blocking cause high background and weak antibody signal during immunofluorescence staining?

I'm working with 40um fresh frozen (non-perfused) sections of rat brain tissue and I'm staining for wisteria lection (immunofluorescence). After staining, my tissue has high background and a weak...

10 November 2020 3,620 7 View

How to separate peak in HPLC?

13 August 2020 2,331 8 View

How to select a Global Climate Model (GCM) for assessing climate change impacts on a watershed?

Following the aim of assessing the ecohydrological impacts of climate change in tropical subhumid watershed of Venezuela, we need to select some climate global models (CGMs). So, we will obtain...

05 August 2020 5,911 5 View

No title

Good morning, dear community, I have a preliminary report from an in silico analysis of non-coding short sequences against the SARS-CoV-2 virus. I'd like some advice about which indexed journals...

18 July 2020 5,365 1 View

Mask for outdoor exercise during covid pandemics?

Hi !! I've seen a lot of arguments in favour and against the use of a mask while running outdoors. I think it's just that some people are not comfortable enoughwearing one, but maybe there is some...

08 July 2020 4,927 2 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

How to calculate the sample size required to detect the difference in pharmacokinetics of methotrexate?

I came across with this study here, https://www.clinexprheumatol.org/abstract.asp?a=5066 And this study says: "The sample size calculation was done to compare two different MTX starting dose...

14 February 2021 3,017 3 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

R/Python package for time-intensity or progressive profiling in sensory evaluation?

Hi I've been looking for a stat package for my sensory evaluation data (progressive profiled which is a multi-timepoint version of descriptive analysis). Is there any available one to...

08 February 2021 9,170 3 View

Is there any publication about the effect of basketball and volleyball on body height?

Many people believe that some stretching sports with vertical jump such as basketball and volleyball can increase the body height. On the other hand, others do not agree with this argument. The...

02 February 2021 281 6 View

After cutting bands having low molecular weight peptides obtained from SDS-PAGE,is it possible to elute these peptide(s) without digesting in trypsin?

Basically, I want to get the sequence of peptides. I assume, digesting these peptides from each band may give me a different sequence than the original through LC-MS analysis. If digestion doesn't...

30 January 2021 5,482 3 View