Methods for intergrating whole blood and PBMC RNA-Seq data?

More Mari Johnson's questions See All

What will happen when we open the model in higher version which created in lower version?

What will happen when we open the model in higher version which created in lower version? Where to add *CONSTRAINT CONTROLS, NO CHECKS in keyword editor? Is there any issues with * Model change...

17 February 2021 3,429 3 View

Bootstrapping: to use or not to?

Hello, I would need help w.r.t deciding on an approach. I sent out a survey and about 2000 of 15000 recipients completed the survey. Doubts have been raised on accepting the results based on...

15 February 2021 5,585 13 View

Crack definition at symmetry plane?

How to define a crack at symmetry plane? Is it right idea? Crack seam we cant specify at symmetry edge (As crack seam can be specified at interior face of 2D). How to handle this type of...

26 December 2020 2,437 4 View

Does cleaning up cDNA after reverse transcription reduce late cycle background?

I've been wondering if removing the random primers from the reverse transcription reaction either with a column or precipitation before using it as a qPCR template reduces the background a bit in...

25 December 2020 6,258 4 View

How to find porosity of a sintered ceramic compact ?

I want to know the porosity of my sintered ceramic composite ? Please help me out

21 December 2020 4,343 6 View

Can I include multiple outcome measures of the same construct, from the same study, in a meta-analysis?

I am carrying out a meta-analysis that looks at cognitive inhibition as an outcome. A wide range of assessment types can be used for this outcome and sometimes a study will include two or three...

15 November 2020 7,055 1 View

What is the meaning and applications of stationary crack analysis especially in Abaqus?

What is the meaning, observations and applications of stationary crack analysis especially in Abaqus? Without crack propagation and crack growth how stationary crack helps in research?

30 October 2020 1,448 4 View

How to extract and segregate SIF data from 3D stationary XFEM Crack automatically?

How to extract data automatically from Abaqus history output request such as SIF of XFEM stationary crack in 3D, from Abaqus to excel or notepad or Matlab with data segregation.? I mean K1 K2 K3...

25 October 2020 9,652 5 View

Can anyone suggest me an industry working in Biostimulants ?

it would be very helpful if anyone would suggest industries working in biostimulants.

25 October 2020 5,824 2 View

How can we confirm the formation of solid solution by using EDX point analysis and elemental mapping ?

I fabricated a composite say AB and both are carbides ceramics having formula AC and BC. I did EDX point analysis and observed A (45 wt %) and B (28 wt %), Carbon (26 wt %) and Oxygen (1 wt %)....

20 October 2020 7,419 7 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Deepyaman Das

For cell specific studies go for single cell RNA seq data for PBMCs

Péter Gyarmati

If you have single cell data, something like this might work to isolate neutrophils/eosinophils:

https://www.nature.com/articles/s41592-020-0825-9

Depro Das

It is better to consider batch as a factor in the design formula. The tximport pipeline proposed by Michael Love himself offers the most useful solution. Please have a look.

https://bioconductor.org/packages/devel/bioc/vignettes/tximport/inst/doc/tximport.html