Is it possible a DNA with an absorbance ratio A260/A230 = 2.96, for example, be influencing the low fluorescence in the Real-Time PCR? I was trying to optimize a real time PCR method to amplify a specific segment and for this purpose, I was using primers which were designed for a conventional PCR and the fragment lenght is 200bp. Could be this the problem which is causing the low fluorescence?

I've already used different SybrGreen Master Mixes...

More Mónica Rodrigues's questions See All
Similar questions and discussions