Mónica Rodrigues

2 Questions 3 Answers 0 Followers

Questions related from Mónica Rodrigues

Someone had ever tested plasmid quantification through Digital PCR with SYBR Green?

Can you tell me if the insert length can influence the PCR reaction? Usually we should use digital PCR (as it is common in real time PCR) for 100-200bp fragments. Is it possible to quantify a...

20 July 2016 569 3 View

Does anyone know the best DNA extraction method of maize stems and roots?

Is it possible a DNA with an absorbance ratio A260/A230 = 2.96, for example, be influencing the low fluorescence in the Real-Time PCR? I was trying to optimize a real time PCR method to amplify a...

23 March 2016 3,606 3 View