Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

The use of n-of-1 studies for rehabilitation / therapies (i.e. non-drug/medicine studies)?

Hi there, I am eager to hear from researchers or clinicians who have used n-of-1 studies for the above purposes. They seem to offer advantages over many other study designs, particularly for when...

26 October 2020 328 5 View

PLS-SEM or CB-SEM after qualitative work?

Hello, My research team completed extensive interviews/triads with participants on various topics. The quantitative survey is the next step in our process. From that survey we want to prioritize...

13 August 2020 828 5 View

Are there examples of non-native insects that respond negatively, or neutrally, to anthropogenic disturbance?

I've combed the literature, but am having a hard time finding any examples. Specifically, I am looking for any examples of non-native insects that respond negatively, or neutrally, to...

30 July 2020 8,920 6 View

Nanotechnology question. Getting rid of solvent after LNP formulation?

I'm dealing with really micro volumes and I'm trying to figure out if there are other efficient methods aside from dialysis to get rid of residual solvent for LNP purification. And if not, are...

25 May 2020 6,377 1 View

Why is SDS-PAGE incorporated into Western Blot over native PAGE?

I know both can be used, but why is SDS-PAGE "preferred"? Does it have to do with cross-reactivity?

29 March 2020 6,000 5 View

Do anyone have the experience of testing creatinine (kidney function) by using HPLC?

In my experiment, I want to use serum (mouse) to test the creatinine level via HPLC. 1. During the preparation, I centrifuged the whole blood, and then transfer the supernatant (which is serum)...

17 February 2020 9,679 2 View

If two drugs activate the same apoptotic pathways, can they have an antagonistic effect?

Hello! I'm testing the photochemotherapeutic potential of a drug in vivo. It turned out that treatment with the prodrug caused an effect that was similar to the effect obtained after...

10 February 2020 3,001 3 View

How do you conduct a factor analysis with Multiple Imputation Output in SPSS?

I am working on my doctoral dissertation and have hit a road block with my factor analysis. I used multiple imputation for missing data (n=198 with 5% missing data), but now I am unable to figure...

05 February 2020 6,437 3 View

Why does my analysis keep excluding one of the classes?

We are analysing data on a cluster randomised control trial. We have treated the clusters (classes, N = 8) as fixed effects, thus dummy coding to create 7 different variables. This is because we...

04 February 2020 9,301 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View