In-house ELISA too much signal. Any suggestions ?

09 November 2020 5,509 5 View

Hi everyone, I am considering to buy pH3000 research grade pH sensor and electrode from Sensorex. Has anybody used that before?

If not, any recs for a good pH sensor to use in the lab for in vitro assays? Thank you so much

20 September 2020 7,416 1 View

Is this journal a serious journal?

Hello everyone, I want to know your opinion about: "journal of musculoskeletal and neuronal interactions" is it a serious and real journal (not a predatory one) to publish in? Thanks

25 July 2020 3,806 4 View

What is the best solvent/method to be use to prepare PVB (Polyvinyl butyral) solution?

I would like to spin-coat a thin film of PVB - the PVB is in powder form - which solvent should I use to prepare the PVB solution and what are the process to be followed? Thanks

04 February 2019 8,677 15 View

Could anyone tell me how to find refractive index of SiO2 thin film, from its UV/Vis transmission spectrum?

I have recorded UV/Vis transmission spectrum of SiO2 thin film deposited on glass. I want to find the refractive index of the film. My transmission spectrum does not represent interference...

01 June 2018 5,500 5 View

I tried several times to get an ethanolic extract for a plant powder but the extract was very little after evaporating ethanol?

I tried several times to get an ethanolic extract for a plant powder but the extract was very little after evaporating ethanol? what is the reason

06 February 2018 3,462 9 View

Is remote sensing data an alternative of field readings?

The use of remote sensing for the assessment of a aquatic resources provides several advantages compared to conventional approaches for water quality assessments. So, can we replace the field data...

04 February 2018 3,269 31 View

Where do I find data on global mean annual Lake surface temperature?

01 March 2017 977 3 View

Does chemotherapy affect RNA quality and quantity after its extraction from tissues?

15 January 2017 5,413 2 View

How to reconstruct high dimensional space from low dimensional space?

22 March 2016 9,168 3 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

How to get rid of somebody pretending to be the author of a publication?

One of the publication I co-authored has the author's name in abbreviation. Then, somebody whose nothing to do with that paper acquired it as her work. To whom in this RG shall I ask for a hand,...

27 February 2021 4,206 4 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Why is My firend's profile not visible?

Hello, I created a profile for my friend on google scholar. I have added a verified email address. I marked the profile as public. Formerly his profile was visible. However, now, When I type his...

24 February 2021 8,680 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View