In-house ELISA too much signal. Any suggestions ?

09 November 2020 5,509 5 View

Hi everyone, I am considering to buy pH3000 research grade pH sensor and electrode from Sensorex. Has anybody used that before?

If not, any recs for a good pH sensor to use in the lab for in vitro assays? Thank you so much

20 September 2020 7,416 1 View

Is this journal a serious journal?

Hello everyone, I want to know your opinion about: "journal of musculoskeletal and neuronal interactions" is it a serious and real journal (not a predatory one) to publish in? Thanks

25 July 2020 3,806 4 View

What is the best solvent/method to be use to prepare PVB (Polyvinyl butyral) solution?

I would like to spin-coat a thin film of PVB - the PVB is in powder form - which solvent should I use to prepare the PVB solution and what are the process to be followed? Thanks

04 February 2019 8,677 15 View

Could anyone tell me how to find refractive index of SiO2 thin film, from its UV/Vis transmission spectrum?

I have recorded UV/Vis transmission spectrum of SiO2 thin film deposited on glass. I want to find the refractive index of the film. My transmission spectrum does not represent interference...

01 June 2018 5,500 5 View

I tried several times to get an ethanolic extract for a plant powder but the extract was very little after evaporating ethanol?

I tried several times to get an ethanolic extract for a plant powder but the extract was very little after evaporating ethanol? what is the reason

06 February 2018 3,462 9 View

Is remote sensing data an alternative of field readings?

The use of remote sensing for the assessment of a aquatic resources provides several advantages compared to conventional approaches for water quality assessments. So, can we replace the field data...

04 February 2018 3,269 31 View

Where do I find data on global mean annual Lake surface temperature?

01 March 2017 977 3 View

Does chemotherapy affect RNA quality and quantity after its extraction from tissues?

15 January 2017 5,413 2 View

How to reconstruct high dimensional space from low dimensional space?

22 March 2016 9,168 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View