Does anyone know which tubes the PAXgene Blood RNA Tube corresponds to? Are they EDTA tubes or Heparin tubes?
Best regards,
Hind Sebbah
they are their own tubes with proprietary preservative in them. they should be collected last at the draw
Interested in enhancing your scientific contribution? Please join us and contribute to book chapters in one of our books published by Springer, Elsevier and CRC Press. Please contact me on:...
04 June 2024 6,827 0 View
Dear researchers and scientific authors, 📚 We are currently collecting contributions for a book on Biomass waste towards sustainable industrial applications that will be published by Springer in...
04 May 2024 7,577 8 View
The aim is to gather more scientists and Ph.D students working on different scientific topics, to share knowledge about current researches, to develop scientific network for further works as well...
07 February 2024 7,451 4 View
Hello! Is it possible to identify pigments using HPLC-MS but without the use of any standards? Despite not having any pigments standards, I have performed the separation of my extracted pigments...
04 February 2024 2,200 4 View
I recently started using Design-Expert. I've entered the response data (response = rejection rate(%)) and have obtained a model equation (the chosen model is a quadratic model, and the ANOVA test...
29 January 2024 4,499 0 View
Dear researchers, For our upcoming book entitled 'Nanocellulose Unveiled: Market insights, Production, and Global industrial Applications', we're looking for authors to contibute in book...
20 January 2024 7,212 2 View
Dear researchers and scientific authors, 📚 We are currently collecting contributions for a Handbook of Sustainable Industrial Wastewater Treatment that will be published by CRC Press/ Francis...
17 January 2024 3,774 9 View
Dear researchers, I would like to ask you if anyone have a cracked version of Graphpad for Mac. Because I'm absolutely needing it, I don't have the free version anymore. Best regards Hind Sebbah
24 December 2023 5,708 0 View
Dear researchers, I am currently culturing THP-1 cells in a T75 flask. I would like to inquire whether it is possible to achieve a confluence of 100% with 32 million THP-1 cells in 20 ml of...
03 December 2023 8,050 2 View
Dear researchers, I have isolated PBMCs from whole blood using EDTA tubes with Lymphoprep. I followed the protocol, but I have come across some forums suggesting the dilution of blood with RPMI,...
25 November 2023 9,953 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
Is this circle one of the rings?
03 August 2024 5,988 0 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View