What are your tips to write the discussion section of your scientific work in an efficient way?

Writing the discussion section is always the scariest part of a paper or a thesis. I came up with some ideas to make it less difficult, but would like to hear other tips! Here are my two bits:...

04 November 2020 6,808 3 View

How can I determine the total antibodies in the serum of bovines with ELISA?

I want to determine if there is a difference in the amount of total antibodies in the serum of different bovines, I was thinking of doing it with Elisa sandwich. Has anyone tried it? What initial...

13 August 2020 2,412 4 View

What measures have your labs/ institutions put in place to mitigate the impact of COVID-19 on early career-researchers?

Early career-researchers are disproportionately affected by the COVID-19 pandemic in academics, as they often struggle with precarious working situations and they are seeing many doors shutting...

27 May 2020 1,440 1 View

Avigan ( Favipiravir), Hydroxychloroquine plus Azithromycin. A possible Cocktail against SARS-COV2 ?

All the three medicaments have promising results reported and supposed complementary mechanism of action?

10 April 2020 6,909 7 View

In Limma package, why should I linearize my data in some analyses?

tl;dr: Why data linearization is applied in the identification o differentially expressed genes/proteins? I am new to data analysis of big data like proteomics. As far as I know, a simple t-test...

16 November 2019 8,441 4 View

What are the do's and don'ts of good research dissemination plans?

Funding agencies increasingly ask for research dissemination plans, in particular in public health, and some require that a considerable amount of time in your project is solely dedicated to this...

17 October 2019 7,557 3 View

Is there anyone who works with ImageJ/Skeletonize3D for quantifying microglia morphology from photomicrographs?

I am having some troubles at the end of the analysis and I guess is due to my version of ImageJ 1.45S. Which version of the plugin Skeletonize3D and AnalyzeSkeleton should I use? I have tried a...

05 April 2019 632 2 View

Could we organize collective human intelligence to achieve and surpass the breakthroughs obtained or crystallized by individual human geniuses?

The biophysicist Jacques Monod hypothesized that man will never understand itself due to that a logical system could not be higher than itself. With the internet becomes common use the somewhat...

30 January 2019 8,014 10 View

Are strain rate effects automatically considered in ABAQUS?

Hi, I'm using ABAQUS to perform a dynamic explicit analysis and I would like to know if strain rate effects in the material behavior are automatically considered by the software. My model involves...

23 August 2018 7,949 5 View

Someone would have a protocol for extracting milk proteins to be analyzed by SDS-PAGE?

I need a protocol that extracts both, whey proteins and caseins, to evaluate the protein profile of proteins present in dehydrated bovine milk by polyacrylamide gel electrophoresis (SDS-PAGE).

30 July 2018 2,151 7 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View