Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

Do you can put again my publications online?

Dear All, Please I has accidentaly removed 22 publications from RG my profile in the mail that you send me today, please put again those publicatios in my profile. Best regards,

13 December 2020 9,981 4 View

Can we predict differential expression genes (MicroRNAs) by analyzing a DNA Methylation dataset?

29 July 2020 7,333 2 View

Which CPU to choose for CFD workstation (E5-2680 V3 vs E5-2667 V3 vs ?)?

Hello, I'm evaluating 2 options for a home CFD workstation for similar prices (I've found good deals for about $1,600-$1,700): CPU: 2x Intel Xeon E5-2680 V3 (2.5-3.3GHz) -12C RAM: 64GB DDR4...

22 November 2019 6,632 3 View

What is the most accepted probabilistic criterion considering frequency layers (fire) via distribution modeling?

fire index prediction

15 September 2019 3,112 2 View

Is there any culture media, where one could mimic the infection conditions of S. aureus?

At our lab, we frequently grow Staphylococcus aureus using TSB or Chemically Defined Media. The latter gives us some control on the conditions we want to impose to the organism, but I was...

05 August 2019 1,787 5 View

How can you read cel files using the R program?

27 February 2019 8,863 2 View

Has anyone done EdU injections in young mouse pups?

Hi everyone, For my neurogenesis project, I'd like to inject young pups (P14) with EdU for a long-term chase (P56). So far, my idea was to perform two days of pulse with 2 injections per day (P14...

16 January 2019 7,012 2 View

Could you please suggest some research works about psycology of language?

Dear Sir/Madam, At this moment I'm currently working in my dissertation project. The question is "in which way is the teacher of portuguese as a foreign language conditioned by his culture in...

24 December 2018 5,861 2 View

How can I prevent glycerol adsorption by PDMS after a couple of days in the incubator?

Cheers everyone I'm currently working with a microfabricated device composed by a thin PDMS sheet assembled onto a glycerol-coated glass slide. I need the PDMS to glide over the glass, hence I'm...

19 September 2018 4,705 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

Which is better medicine or food in obesity?

Preference in terms of the scientific aspect

28 February 2021 796 2 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

27 February 2021 9,626 4 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View