Hello all,

I am studying the benefits of a diet in the abrogation of high-fat diet induced cardiac remodeling of the heart. I am studying this in C57BL/6J mice and the study was already conducted and I have the tissues frozen.

My question is: do I extract protein and RNA from both ventricles or just the left ventricle? The left ventricle seems to be more susceptible to remodeling, and since I am evaluating metabolic, fibrotic and hypertrophic markers, extract only from the left ventricle could be the best option. On the other hand, I don't know if I will have enough sample for both extractions and histological analysis.

Thank you in advance.