Hello,

I'm working with FFPE tissues and aiming to perform a proteomic analysis of these samples. I'm really struggling to find a buffer that works for the extraction of proteins before digestion with trypsin. I performed protein quantification using Nanodrop, but it indicated a very low protein amount (used PTS buffer and another one with with urheia, TRIS - did not worked).

The buffer that worked for me was the RIPA buffer, but it contains detergent. How can I remove the detergent during sample preparation? There is any buffer that works for extraction that does not contain detergent in its compossion? Any suggestions?

I am very thankful in advance for any help and future responses.