Thank you for your reading.

I'm working on mouse primary hepatocyte culture.

I've used William's medium E (1X PenStrep, 100 nM Dexamethasone, 100nM insulin, 2 mM L-glutamine, 50 nM glucagon without FBS) for my heptocyte culture.

I understand why we need to add those components in the medium but except glucagon.

I've searched some article about this question but I still don't found my answer..

Is glucagon essential for primary hepatocyte culture?

Since insulin is put in the media, is it necessary to put glucagon as well?

Thank you. and have a good day! :)