10 March 2015 1 1K Report

Preferably a PCR based assay

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Quantifying coal particles in marine sediment?

Can anyone advise on a method to directly quantify coal particles in marine sediment, or an indirect quantifiable measure of their presence? Regards, Brent

16 February 2024 4,639 0 View

What is the best Statistical Tool/Analysis to compare LC50's from different sources?

Data on hand: LC50 per part of Peperomia (roots, leaf, stem) against various cancer cell lines (lung, cervical, breast) LC50 of controls against various cancer cell lines (lung, cervical,...

07 December 2021 6,484 3 View

What would explain producing only the template after FastCloning?

I am having trouble figuring out how my results after FastCloning show that only the template was amplified. I have three sets of primers that are designed to insert anywhere from 10-12 AA's at...

11 March 2021 1,707 1 View

Case Studies on Church Revitalization?

I am seeking Case studies on Church revitalization. Any and all kinds of research will be helpful. Case studies focusing on one church, or case studies that focus on a comparison of churches in...

25 October 2020 7,555 4 View

How to calculate a gradient of dissimilarity across multiple sites using presence/absence data?

I’ve been trying to come up with a metric for species (dis)similarity for fish in a series based on presence or absence data that gives a value of 1 or 0 for each lake. From my research, it seems...

04 February 2020 1,983 1 View

Can anybody help with identifying some spirorbid tubes?

I am currently working on the meriofauna (63 - 2000 microns) on the calcareous algae Corallina officinalis in west Wales. I am at some sites turning up numerous spirorbids. This is a group new to...

08 January 2019 9,799 0 View

Need help deciding on correct test to use for species richness at two (or more) sites?

I need to test whether the composition of species richness is different between two (or more) sites (not just the difference between the total number of species). I have presence or absence (1, 0)...

20 November 2018 3,552 2 View

Can Raji cells produce infectious virus?

I've read that TPA can induce lytic genes in Raji cells but is cell free virus infectious? Induction of Epstein–Barr virus (EBV) reactivation in Raji c...

06 November 2018 2,736 1 View

FITC labled Primary Antibodies for one step Western Blots?

Will FITC labeled primary antibodies still bind their epitope (phospho-Tryosine in this case) in western blotting? Assume an unlimited supply of primary antibody. I feel like this would save time.

11 September 2018 7,629 2 View

Do I need to use Brij-35 when working with MMP-2?

I am wanting to conduct an in vitro degradation study of some hydrogels in the presence of the enzyme, but I don't have Brij-35 in stock. Am I able to use a different surfactant, such as Triton X-100?

07 August 2018 732 1 View

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

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Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

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Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

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Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

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Are the apoptotic cells is positive for γH2AX ?

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Can anyone help me explain my problem?

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Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

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