We haven't been successful at detecting NLRP3 in THP-1 or monocytes, and get a very messy blot with no clear NLRP3 band. Thanks!
Hi, I used the NLRP3 from Enzo. It works in human macrophages. There are some non-specific bands but works very well in immuno-fluorescence staining
Good luck
Try from Thermo Fisher. It works really well in immunoblotting.
Thank you!
I always use a monoclonal antibody from Enzo (ALX-804-819-C100) and works great for IP and WB
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