I work with murine intestinal organoids and recently our batch of homemade R-Spondin doesnt seem to be working. I'm looking forward to recommendations for a commercial rspondin that works.
Hi Tharini, I am culturing intestinal organoids since 3.5 years. I tried R-Spo from Peprotech and it worked: recombinant Human R-Spondin-1 (Pepro Tech, catalog number: 120-38). http://www.bio-protocol.org/e1125
Thanks for the info :) I 've already used the one you've mentioned. :)
Also, these days I am using the INTESTICULT medium from Stemcell technologies. It is working very well for growing organoids and is quite affordable! :)
I can vouch for the Peprotech product mentioned by Agnieszka. We use it in our lab, though we also use the conditioned media a lot of the time.
Can I ask what you think is the issue with your conditioned medium? Because if you are using any quantity of RSpo1 the conditioning route is far more cost effective.