According to the paper by T Sato et al, 2011, they used human recombinant R-spondin from Nuvelo

Article Long-term Expansion of Epithelial Organoids From Human Colon...

Thank you for your reply Noha. I will look into it

Hi Tharini, I am culturing intestinal organoids since 3.5 years. I tried R-Spo from Peprotech and it worked: recombinant Human R-Spondin-1 (Pepro Tech, catalog number: 120-38). http://www.bio-protocol.org/e1125

Hi Agnieszka,

Thank you for this information. I will look into this right away :) 

Cheers,

Tharini 

Hi Tharini,

If you are still looking for an R-Spondin 1 for your organoid cultures, R&D Systems has a Recombinant Mouse R-Spondin 1 available.

https://www.rndsystems.com/search?keywords=R-Spondin%201&species=Mouse

Best of luck,

Scott

https://www.rndsystems.com/search?keywords=R-Spondin%201&species=Mouse

Hi Scott, 

Thanks for the info :) I 've already used the one you've mentioned. :) 

Also, these days I am using the INTESTICULT medium from Stemcell technologies. It is working very well for growing organoids and is quite affordable! :)

Thanks again,

Best,

Tharini 

I can vouch for the Peprotech product mentioned by Agnieszka. We use it in our lab, though we also use the conditioned media a lot of the time.

Can I ask what you think is the issue with your conditioned medium? Because if you are using any quantity of RSpo1 the conditioning route is far more cost effective.