In mouse SI-lamina propria T lymphocytes stimulation with PMA (20ng/mL) /Iono(1ug/mL) is not strongly inducing IFN-g after 6hrs. The previous results in our lab had 4-5% and I could have just 2% max, though the protocol and antibodies to stain everything is followed in same way! any suggestions or comments would be greatly appreciated.

Bref A (1ug/mL) after 2hrs of PMA7Iono.