When to include covariates?

Hi it's me again! Coming to you with a question about including covariates in a model. So I'm running a mixed effect model in my longitudinal imaging data. I have 4 possible covariates to...

11 February 2021 1,404 1 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Prediction of novel precursor miRNAs in viral genomes?

Dear, I performed a multi-sequence alignment (MSA) with all the complete genome sequences of SARS-related viruses. I then used RNALalifold from the ViennaRNA package, with default parameters, to...

06 December 2020 1,704 3 View

SPSS ANOVA with 2 groups, 3 time points and hundreds of dependent variables?

Hello everyone! I need to run ANOVAs for multiple variables that take place at 3 time points. So I have 2 groups (controls and patients), 3 time points for each measure, and then a plethora of...

22 November 2020 8,032 5 View

Can I calculate mean and confidence intervals of d-prime values?

Hi everybody. I ran an experiment on memory and calculated the percentage of hits as usually reported in the literature. However, the reviewer asked me to transform my hits into d-prime values. I...

21 October 2020 3,859 1 View

Discrepancy between RNA and protein levels in single-cell seq? CD3 mRNA vs protein?

Hi all, A very quick question. I have sorted human T cells from blood using anti-CD3E, CD4 and CD8a antibodies. We did single cell seq on those sorted cells. Analysing the results, I do not see...

19 September 2020 4,953 3 View

What are the nuclei mean number from freeze tissue nuclei isolation protocol?

Hello, everyone! I'm going to start a project with single-cell ATAC-seq for analysis of open chromatin regions from freeze tissue samples. I'm lost about the nuclei concentration. If I made a...

19 June 2020 7,348 2 View

How do I compute for the Pr(Y=1|X=1) H1 and Pr(Y=1|X1) H0 for a priori estimation of required sample size?

I am trying to estimate the required sample size replicate a study which used a probit regression analysis. The paper reported the probit regression results and the marginal effects but I can't...

17 May 2020 4,826 1 View

How can I measure learners' overall level of "investment" (Norton,2015) quantitatively?

I would like to investigate students' level of investment (not motivation) in an English class. are there any questionnaires I could use for this?

24 March 2020 6,940 3 View

How do you deal with your enironmental chemistry data?

Hi everybody! I am PhD in analytical chemistry and I make part of a multidisciplinary team working on the development of a web based solution focusing mainly on treatment and management of...

12 March 2020 5,597 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View