Ariana Bravo Cruz

2 Questions 2 Answers 0 Followers

Questions related from Ariana Bravo Cruz

Does anyone know of a cell line (from C57BL/6 mice) that can induce lymphomas (preferentially NHL) when introduced in SCID mice?

Similar to BJAB (human) or A20 (balb/c) but a C57Bl/6 one

08 May 2018 4,017 2 View

For looking at T cell proliferation and cytokine expression. Can I use splenocytes (then gate on T cells) or do I need to work with isolated T cells?

I am going to be looking for IL-4 and IFNg by ICS and proliferation by Ki-67 expression by flow after ex vivo stimulation with CD3 (5ug/mL-coated plate)+ soluble CD28 (2ug/mL) or PMA (50ng/mL) +...

08 February 2018 6,784 2 View