Seems like even if virus works with young animals expression is pretty low in aged ones...but maybe there is something better and preferably not pathogenic...some AAV serotype that may work with aged brain or something? Or perhaps nothing like that actually exists if you take into consideration that with aging protein production and degradation pathways are dysregulated. What is your opinion?
aav9 works well till 9 months of age in mice, I have no experience later. rabies is very effective at all ages. I wonder wether the sindbis would work...
Not hippocampal, but we have worked extensively with CamK II positive excitatory neurons in the amygdala, working with adult males. Here is a link to the study; http://www.biomedcentral.com/1471-2202/15/28 . We find that our pseudotyped AAV2/7 and AAV2/DJ8 are very efficient at transduction of these cells. We should soon have more data to share reinforcing these conclusions.
Yeah we have tried AAV9 and it works great in 3-6 month old rats but in our 30 month old rats its very variable.