Does anyone know if the peptides provided with/against some santacruz antibodies will work also for elution or is it only for competition studies?

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What is the most optimum text classification algorithm to predict stock market ?

Data mining

20 October 2016 7,484 5 View

Is there any text-analysis software that can help analyse cohesive devices (other than cohmetrix)?

Cohesive devices include reference, ellipsis, substitution, conjunctions and lexical reiteration.

17 December 2014 5,722 4 View

Is there any text-analysis software that can help analyse cohesive devices (other than cohmetrix)?

Cohesive devices include reference, ellipsis, substitution, conjunctions and lexical reiteration.

11 December 2014 6,923 5 View

Does anyone have knowledge on convergent parallel and embedded mixed methods research?

I collected my data from three medical settings in one phase time where I conducted non-participant observation (i.e. medical consultation was recorded), then a questionnaire that patients had to...

06 July 2014 8,995 22 View

Do you know which Statistical test could be used to examine the effect of caregiver's gender on patient satisfaction?

The effect of caregiver's gender on patient satisfaction.

25 December 2013 1,993 4 View

Doctor-patient-caregiver alignment

I am working on doctor-patient-caregiver alignment from a conversation analysis perspective. Has anybody done the same before? Actually I am facing difficulty in finding enough materials in this...

22 January 2013 5,247 4 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

15 July 2024 6,008 5 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View