Sharad Purohit

5 Questions 90 Answers 0 Followers

Questions related from Sharad Purohit

Protocol for to prepare a melibiose immobilized on bead support?

Hello, I am searching for a protocol to create a immobilized melibiose column to purify anti-alpha-Gal antibodies from human serum. the crosslinked guar gum column described here Preparation and...

22 May 2023 827 0 View

Why do humans and animals produce antibodies against simple sugars?

I am measuring anti-carbohydrate antibodies in human serum and noticed that there is a large amount of antibodies against simple hexoses in the serum. Why humans produce these antibodies against...

14 August 2014 2,841 5 View

How to align the gene sequence in the plasmid for bacterial expression?

I would like to clone a cytokine in pet32 vector. The gene sequence was downloaded from NCBI and I checked the coding region for protein. How do I make sure that the gene is in alignment with the...

13 September 2013 4,420 2 View

What is the cellular composition of a spleen of a mouse?

Hello, what is the percentage composition of CD3+ cell CD4+ cells CD80+ cells CD86+ cells CD11c+ cells in spleen of a mouse

08 July 2013 1,729 1 View

Isolating IP products using gel-filtration.

I would like to isolate the IP products using human serum as a source of antibodies. Several papers report the use of sepharose 6b columns to fractionate the IP products. I am looking for a quick...

29 March 2013 3,608 2 View