I am working on a plant with high polysaccharide content. For genomic DNA extraction, we have used several protocols but failed to get a good quality DNA. We found out that the STE/CTAB approach might be useful (Shepherd & McLay, 2011). For preparing STE the following materials are needed (0.25 M sucrose, 0.03 M Tris, 0.05 M EDTA). However, we still do not know the amount (gr/mg) of the material we should take to prepare STE. Does anyone have any experience?
https://agriculture.uq.edu.au/files/5650/plant-genomic-dna-extraction-by-ctab-2-fiona.pdf
Dear Bhairavi Vajaria ,
Thank you very much for your reply. it is helpful. however, there is no sufficient info how to prepare the STE!
Hi Dr. Moazzeni
I found something for you:
http://www2.kumc.edu/soalab/LabLinks/recipes/ste_gdna.htm
http://www2.kumc.edu/soalab/LabLinks/protocols/genomhm.htm
I hope it will be helpful.
Best Regards
Dear Dr. Eslami,
Thank you very much for your help. I have already seen it. Sucrose is missing in this protocol.
Your welcome, not at all.
Would you please tell me the amount of sucrose?
Regards
Then, perhaps it will be useful:
Article Safer DNA extraction from plant tissues using sucrose buffer...
This method consists of an initial washing step with STE buffer (0.25 M sucrose, 0.03 M Tris, 0.05 M EDTA)
Kind Regards
- Badges
- Science method