Try adding good amount of ice cream in the medium, daily!  ;)

There really isn't any such thing as "diabetic cell lines".

Because of differences between T1D and T2D, you have to choose your options and mimic the conditions ex vivo to which your target cells will be exposed. Whatever parameter you are interested in studying will have to be compared with and without your test concoctions.

If you are interested in seeing the effect on insulin secretion from beta cells, you will be using either islets or immortalized cells that secrete insulin in response to increasing levels of glucose. The latter are not as good as islets but isolation of consistently good quality islets takes some experience.

If your interest is more along the lines of various aspects of T2D and influence of your concoction on for example, lipid metabolism, you would probably choose a hepatoma or fat derived cell line. 

Try adding good amount of ice cream in the medium, daily!  ;)

There really isn't any such thing as "diabetic cell lines".

Because of differences between T1D and T2D, you have to choose your options and mimic the conditions ex vivo to which your target cells will be exposed. Whatever parameter you are interested in studying will have to be compared with and without your test concoctions.

If you are interested in seeing the effect on insulin secretion from beta cells, you will be using either islets or immortalized cells that secrete insulin in response to increasing levels of glucose. The latter are not as good as islets but isolation of consistently good quality islets takes some experience.

If your interest is more along the lines of various aspects of T2D and influence of your concoction on for example, lipid metabolism, you would probably choose a hepatoma or fat derived cell line. 

Thanks Tausif Alam, i like the ice cream part!

hi, could you please describe which type of diabetes you want to induce and the type of cell line you are going to handle? If its type 2 diabetes I suggest you to go for insulin resistance in vitro model on insulin targeted cell lines like skeletal muscles, hepatocytes, adipocytes cultured in high glucose environment that leads to hyperglycemic condition. For type 1 diabetes, Pancreatic beta cells can be used. For more, plz refer http://altweb.jhsph.edu/altex/27_2/altex_2_10_skelin.pdf

https://www.researchgate.net/post/Is_it_possible_to_establish_diabetes_condition_in_cell_culture 

" i like the ice cream part!"

Indeed, who doesn't; after feeding your cells with sugar and fat both, there is always going to be leftover which can be undoubtedly put to good use!  ;)

PS: Our university has a facility for dairy research and they make some of the best ice cream. When our children were younger, I used to bicycle there on the weekends and they always enjoyed it. [I was assured that the milk came only from "normal" control cows. Let's hope no one made a mistake of mixing up samples.  ;) ]

What is diabetic cell lines ? do you mean to induce dysfunction in insulin receptors on  the membrane of the cell ? If so you have to occupy all insulin receptors with insulin antagonist molecules.

LY294002 is a selective phosphatidylinositol 3-kinase (PI3K) inhibitor can be used to induce insulin resistance because of their efficacy to inhibit cellular glucose uptake. PPAR gamma antagonist inhibits the transcription of insulin receptor might also be helpful. You can also test the inhibitors of ion channels to block the depolarization of pancreatic cells.   

Sita,

If you search through published literature, you will find that there are very examples of "specific" targeted synthetic inhibitors in a living cell; LY294002 is no exception.

All such molecules muck up many more things. And even if your claim was true, which isn't, PI3K in not only involved in insulin secretion.

But that's not to say that with a clear understanding of limitations of synthetic inhibitors, they cannot be used as useful tools.