Any advise for a glass or transparent plastic with a 2 photon fluorescence properties ?

Hello, I would like to use a transparent material for beam profile imaging while focusing with an objective. The objective is to charaterize the aberration introduce by the index mismatch when...

26 February 2018 3,106 2 View

Subcellular fractionation- why do I get smear in membrane fraction only?

We are running subcellular fractionation of transfected cells with our protein in question to determine its localization. The protein is HA tagged and we are running the fractions on a western...

13 November 2017 2,444 7 View

Are there shared TCRs in tumor bearing C57Bl/6J mice?

I wonder wether there are shared TCRB-amino acid sequences in tumor bearing C57Bl/6J mice. We performed an immunoSEQ assay and found no overlap between our mice regarding the TCR repertoire of...

03 January 2017 5,078 2 View

Which statistical test suits best?

Hello everyone, I'm currently trying to find out which statistical test I can use to show differences between two or more populations. I had cells, which I infected with a virus +/- additional...

27 May 2016 6,675 5 View

Why has my transient transfection stopped working?

Hello I've been transfecting HeLa cells with an rtTA plasmid that contains GFP and my protein of interest. I use Xfect kit from clontech (it's polymer based. you transfect, change medium after 4h...

14 February 2016 2,446 4 View

What is energetically more favorable?

Hey, I've got a question which might be very simple. I was asked which interaction of amino acids is more energetically favorable. My problem begins with the what the definition of "energetically...

31 January 2016 2,964 6 View

How do you calculate annealing temperature for bisulfite primer?

20 January 2015 5,438 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View