As you know vitamin D is not water soluble, I need use it in cell culture media that is watery , can you help me?
You can prepare a stock solution at a concentration such 1000X in DMSO or in another solvent. Then add to your culture medium.
many Thanks, but making Michelle is take times, Is there any good and reliable method for making it just by solution ?
Bio-Tech Pharmacal is an example of companies producing a Vitamin D powder - which dissolves in water.
Dear henry , thanks so much,hope to find that product here, because the company is in USA and I don't know is there any company for entering the products here or not?
It is recommended to use the appropriate quantities (dose-ranging) of 1,25()H)2D3 for cell cultures, rather than using parental D or 25(OH)D to obtain right results and consistency. Almost all cell have 1,25(OH)2D receptors.
Dear sunil , thanks, is the vitamin D in the capsule in the form of 1,25()H)2D3? what do you think about using the capsule of vitamin D for cell culture?
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