I have rare cells in 10 microliter volume and I want to count them by neubaur chamber in 10 microliter volume
As you know this method gives the cell count in ml volume but I won't dilute my cells in ml, is there a formula for this condition?
Hi Fatemeh, if you have a low cell concentration in 10µl, your problem could be that you will find only a few cells in the central 5 x 5 square. Such a low count will cause a poor statistic and hence a relatively large error for your cell concentration. I would therefore also count the cells in the sorrounding 8 large squares (which are usually subdivided in 4x4 smaller ones, see fig6 in the link below). The central (5x5) square and the 8 surrounding (4x4) squares all have the same volume. If you count the total number of cells in all these 9 large squares, you have to divide this number at the end by 9 (as explained on page 5 of the attached pdf manual).
To account for the volume per large square, you normally have to multiply the counted cell number with 10.000 to get the cell concentration/ml. If you want to know the cell concentration per µl, simply multiply the cell count with 10 instead of 10.000.
godd luck, Michael
http://www.celeromics.com/en/resources/docs/Articles/Cell-counting-Neubauer-chamber.pdf1oO5t-fXFzqtghuQ
I think the link on the above message was corrupted. Please use the updated link below for the Neubauer chamber manual.
regards, michael
http://www.celeromics.com/en/resources/docs/Articles/Cell-counting-Neubauer-chamber.pdf
Hi, Fae,
Neubauer chamber needs about 10 microliter cell suspension, not more. So, your task is possible to do!
Greetings,
E.F.
Dear E.F.
my question a bout total concentration of cells per microliter not about volume of chamber...
as you know neubaur give the cells concentration in ml but the total of my cells in 20 microliter not in ml
i wanna know has any formula for calculate cells per microliter
anyway thanks a lot for Ur kindly respond :)
For microliter you should divide ml concentration to 1000. 1 ml = 1000 mkl, therefore. Or multiply the count in big square to 10 instead of 10 000. I normally dilute suspension enough and count the whole chamber, then multiply to 1111 and to dilution. So, for 10 mkl cells number you should multiply the whole chamber cells number to 11, or if you count one of ten small clear-striped squares - to 111.
Formula for Neubauer chamber cell concentration calculation is the same. And the advantage of this chamber count method is 1ml cell suspension is not necessary like for Beckmann particle size/number counter, 10 mkl is enough!
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