Fatemeh Etezadi

16 Questions 37 Answers 0 Followers

Questions related from Fatemeh Etezadi

How to Prepare Single Cardiomyocytes for Flow Cytometry Analysis?

Dear all, I hope you are doing well! I am working with cardiomyocytes (CMs) that differentiate from pluripotent stem cells. I am currently struggling to obtain single cardiomyocytes for flow...

13 February 2025 4,293 0 View

Is there any suitable protocol for cell culture on the glass bottom plate?

Hello everyone. I am going to analyze differentiated cardiomyocytes (CM) via confocal microscopy. However, I struggle to attach CM properly to the glass slide/plate. Although I coated the glass...

24 October 2022 7,425 6 View

Is there any free as well as easy software for counting cardiomyocytes beats?

Dear colleagues, I have several videos from hiPSCs derive cardiomyocytes, which were recorded on 30fp. I want to count their rhythm and compare them to each other. I can not use MYOCYTER or...

23 December 2021 5,200 3 View

Does anyone know what the best and easiest protocol for cardiomyocyte differentiation from hiPSC is?

I tried cardiomyocyte differentiation several times with B27-insulin but it didn't work I used 6 micromolar CHIR for wnt activator on day 0 (for 24h) and 2 micromolar XAV939 as a wnt inhibitor on...

10 October 2019 8,775 6 View

Are there any scaffolds with controlled growth factors release for tissue engineering in the market yet?

which company/institute make scaffolds with controlled growth factors release ?have any hospital or clinical trial used of this approach for tissue repair?

10 April 2016 7,469 4 View

Who can give me some information about DRPINs?

Are there companies that sell DARPins?Are there any websites that describe how to design DARPins?

10 October 2015 4,274 2 View

Does anyone know how can i count cells in microliter volume not in ml by use of neubaur chamber? Is it possible or not?

I have rare cells in 10 microliter volume and I want to count them by neubaur chamber in 10 microliter volume As you know this method gives the cell count in ml volume but I won't dilute my cells...

23 February 2015 4,909 5 View

Who can send me the photo of normal hematopoitic stem cells which isolated from cord blood?

I wanna know the morphology of them

10 March 2014 6,250 1 View

How can I separate the viable cells from the dead cells in suspension culture?

i'm working on MOLT-4 (acute lymphoblastic leukemia). It grows as suspension and I want to know more information about which is the best culture method to separate viable cells from dead cells.

28 December 2013 1,132 18 View

How can I conjugate an antibody to iron oxide nanoparticle?

I wonder if you would mind me offering a protocol about conjugation of antibody to IO nanoparticle for cell separation

17 December 2013 3,837 3 View

Which company produces specific antigen?

I want to order some antigen and want to know which company is best?

18 November 2013 5,719 3 View

Does anyone know which cancer cell lines (Cancer stem cell, tumor or metastatic) are positive in EpCAM?

I wanna set up a control for my test I want to use some cell lines that the cells are positive in EpCAM.

25 September 2013 9,072 1 View

Have you ever coated cell culture plates with an antibodies for capture some cells?

If not, is it possible? How? Could you send me some article that do this approach for capture the cells in cell culture plates?

23 September 2013 9,451 3 View

Which growth factors in culture medium can be use that are the best one for cancer stem cells or circulating tumor cells?

I want to culture CTCs and I want to make a medium that contains some growth factor that specific for CSC or CTC. Actually I want to get rid of another cells in culture medium and all the cells...

09 September 2013 9,618 1 View

How can I coat the antibody on the surface of the plate manually with routine tools?

I want to coat an Ab on the surface of a plate (actually like plate of cell culture). Also, how can I detach cells that captured without our cells being destroyed?

27 August 2013 7,996 14 View

Which stage of breast cancer patient have the highest amount of detectable CTCs in their blood?

Which group of breast cancer patients have the best sample with the highest CTCs?

24 August 2013 9,366 2 View