16 Questions 37 Answers 0 Followers
Questions related from Fatemeh Etezadi
Dear all, I hope you are doing well! I am working with cardiomyocytes (CMs) that differentiate from pluripotent stem cells. I am currently struggling to obtain single cardiomyocytes for flow...
13 February 2025 4,287 0 View
Hello everyone. I am going to analyze differentiated cardiomyocytes (CM) via confocal microscopy. However, I struggle to attach CM properly to the glass slide/plate. Although I coated the glass...
24 October 2022 7,413 6 View
Dear colleagues, I have several videos from hiPSCs derive cardiomyocytes, which were recorded on 30fp. I want to count their rhythm and compare them to each other. I can not use MYOCYTER or...
23 December 2021 5,186 3 View
I tried cardiomyocyte differentiation several times with B27-insulin but it didn't work I used 6 micromolar CHIR for wnt activator on day 0 (for 24h) and 2 micromolar XAV939 as a wnt inhibitor on...
10 October 2019 8,761 6 View
which company/institute make scaffolds with controlled growth factors release ?have any hospital or clinical trial used of this approach for tissue repair?
10 April 2016 7,455 4 View
Are there companies that sell DARPins?Are there any websites that describe how to design DARPins?
10 October 2015 4,259 2 View
I have rare cells in 10 microliter volume and I want to count them by neubaur chamber in 10 microliter volume As you know this method gives the cell count in ml volume but I won't dilute my cells...
23 February 2015 4,894 5 View
I wanna know the morphology of them
10 March 2014 6,243 1 View
i'm working on MOLT-4 (acute lymphoblastic leukemia). It grows as suspension and I want to know more information about which is the best culture method to separate viable cells from dead cells.
28 December 2013 1,125 18 View
I wonder if you would mind me offering a protocol about conjugation of antibody to IO nanoparticle for cell separation
17 December 2013 3,826 3 View
I want to order some antigen and want to know which company is best?
18 November 2013 5,710 3 View
I wanna set up a control for my test I want to use some cell lines that the cells are positive in EpCAM.
25 September 2013 9,062 1 View
If not, is it possible? How? Could you send me some article that do this approach for capture the cells in cell culture plates?
23 September 2013 9,440 3 View
I want to culture CTCs and I want to make a medium that contains some growth factor that specific for CSC or CTC. Actually I want to get rid of another cells in culture medium and all the cells...
09 September 2013 9,607 1 View
I want to coat an Ab on the surface of a plate (actually like plate of cell culture). Also, how can I detach cells that captured without our cells being destroyed?
27 August 2013 7,983 14 View
Which group of breast cancer patients have the best sample with the highest CTCs?
24 August 2013 9,359 2 View