Does anyone know a good GFP-knockin mouse (GAD67/GAD65) for detection of GABAergic interneuronal migration in embryos (e14.5 - P0)? PV neuronal sub-population is the focus, but the preference is that GFP marks all interneurons. It would be good if the strain is commercially available.
Thanks a lot Michael,
I have thoroughly read the first publication you mentioned a year ago and I consider it to be the bible of HC interneurons.
Do you think that the claim that GAD67-EGFP (Tg(Gad1-EGFP)G42Zjh) has detectable signal as early as E14.5 is false? It is selective for PV interneurons later on, so E14.5 signal (if there) would mark PV subpopulation in development.
http://jaxmice.jax.org/strain/007677.html
I am a bit reluctant to buy this animal thou, still thinking between this and Nkx2.1CRE you mentioned.
Thank you so much,
This was very helpful.
I really appreciate it,
Best,
Marin
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