There are so many analytical methods wtih the combination of methanol/water, acn/water, methanol and acetonitrile, both of three . These mobile phases suitable for RPHPLC methods for the separation of hydrophobic compounds
They can also be used for hydrophilic compounds separation e.g. separation of oligosaccharides using a hypercarb column. We use a gradient 0min 97% water 3% ACN 6min 80% water 20% ACN.