Does anyone know about an ELISA kit that would measure small amounts of human urocortin 2 (UCN2) in serum/plasma?

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Emad Al-Dujaili

Yes I used a kit from MyBioSource. It worked for me but not sure if you can get 10-20pg/mL. Perhaps you can contact them. They have it for most species. Below is an example

MBS919245 (ELISA Kit)

urocortin 2

• Mouse Urocortin-2, UCN2 ELISA Kit; Mouse Urocortin-2 (UCN2) ELISA kit; SRP; UCN-II; UCNI; UR; URP; stresscopin-related peptide; urocortin II; urocortin-related peptide; urocortin 2;

• urocortin-2; Urocortin II; Ucn II;

• 48 Strip Wells .. $540

• 96 Strip Wells .. $775

Theophilus I Emeto

Dear Agnieszka, if you are still searching. may be talk to Uscn life sciences. They may be able to help you out. I have used very good ELISA kits from them previously.

http://www.clinsci.org/cs/126/cs1260517.htm

http://www.uscnk.us/ELISA/ELISA-Kit-for-Urocortin-2-%28UCN2%29-46766.htm

Agnieszka Borys

Thank you Emad and Theophilus for your's answers.

Finally we have used kit from Cusabio and Cloude-Clone and it turned out that the measured values are much higher than expected and vary greatly between the kits (10 fold...). Do you know what could be the expected values of UCN2 concentration in serum/plasma of a healthy donor or where I could find that information? The companies are not willing to give a strait answer, only that the analysis went as expected..

Dear Agnieszka, I have only analysed UCN2 in patients with abdominal aortic aneurysm (AAA) and or peripheral artery disease (PAD) and also in animal models. So I am not sure what the is expected in healthy donors at the moment. However, there is some putative data from Davis and colleagues, the group in Christchurch. Please see link. You could email them directly, they may be able to give you a direct idea what to expect.

Alternatively, the group in Edinburgh led by Venkatasubramanian had conducted a double blind RCT on the vascular effects of UCN2 and 3, so they may be able to give you normal/expected values of UCN2 in plasma/serum. I have also included a link to their publication fro the trial. I hope this helps.

http://www.sciencedirect.com/science/article/pii/S0735109706026945

http://jaha.ahajournals.org/content/2/1/e004267.full

Thank you. I will look at those and perhaps contact those groups but as far as i know they used 'in-house' build ELISA's so I'm not sure if I can directly compare their results to ours.