Does anyone know a ubiquitous and proliferative cell line that is responsive to IL-1?

More Rohullah Jafari's questions See All

If someone says IR sensors are the same as MX sensors, do you accept it?

Somebody has told me Ir sensors that I have used in my circuit, are the same as Mx sensors however they are different and their circuits can not be arranged similar but he does not accept. Is...

06 June 2024 9,117 4 View

Do you have any research or invent about IR sensors?

I want to record a analog circuit between control and power circuits as innovation. My control circuit is IR sensors with bulb size 10mm (its max current to support power circuit is 100m[A] DC)and...

05 June 2024 4,085 4 View

Is it possible to use pair of IR sensors for lighting staircase?

Pair of IR sensors have sender and receiver sensors but some one declares it is possible to use this sensors for lighting stair case however I have used them for automatic liquid dropper and hand...

05 June 2024 8,302 1 View

What is the latest research in the world in the field of personality and artificial intelligence?

22 May 2024 1,070 3 View

How to get copyrights permission for free from Springer nature journals?

I changed the circumstances while ordering, but did not get free access. please help me if you have experience in this issue. thanks

19 May 2024 8,649 1 View

How does early exposure to a foreign language in infancy and childhood affect the unconscious language acquisition process?

Second language learners often experience immersion in real-life contexts with exposure to cultural nuances, diverse input sources, and strong intrinsic motivation. How about foreign language...

02 May 2024 8,307 2 View

Which unsolved problem or question you have in mind about chaos and chaotic systems?

If you are a researcher working with chaos and chaotic systems, which unsolved problem or question you have in mind Here I have collected some open problems and questions related to chaos and...

28 April 2024 8,493 2 View

Is this formulation correct for the linear Drucker-Prager criterion?

If the hydrostatic pressure is defined as p = -1/3 trace(σ), where σ is the Cauchy stress tensor, and under the assumption that Drucker-Prager failure surface circumscribes the Mohr-Coulomb...

13 April 2024 4,416 0 View

Is this expression correct for the linear Drucker-Prager criterion under the conditions stated in the attachment?

Is this expression correct for the linear Drucker-Prager criterion under the assumptions stated in the attachment?

27 March 2024 185 0 View

How can I make sure the topic of my master's thesis is good enough and will be valued later?

I'm currently studying geotechnical engineering. I want to choose a topic and don't have any idea where to start. any suggestion would be appreciated.

30 January 2024 3,897 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View