Does anyone know a highly sensitive method to quantify calcification in mouse blood vessels?

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Deon Bezuidenhout

I do not have experience with mouse vessel calcification or amounts expected, but ashing, dissolving in HCl and and atomic absorption spectroscopy at 423nm works well for heart valve leaflets. regards. Deon

R. John Macleod

Why not do qPCR on Runx and other markers of calcification?

Matthias B. Moor

Thank you, this helped!