Hi Carmine,

If you have access to a flow cytometer, you can do this with multi-parameter staining. Annexin V/Propidium Iodide will give you early/late apoptosis.  An internal stain for cleaved caspase 3/7 (usually both are measured) can be added as it reads in the FL-3 channel.  Flow cytometry is the easiest way.

Annexin V/PI staining is normally performed on live cells via FACS. This will create a problem for you if you want to do surface (Annexin V) and exclusion (PI) staining, while also trying to perform intracellular staining (caspase 3), especially as intracellular staining requires permeablization.