I'm trying to make a co-culture with AGS cells and B cells, and we want to do a sorting. Does anyone here know a good surface marker? this cell line doesn't express catenin beta 1, e-cadherin and vimentin
Dear Jose!
Please You look at the following article:
Article Identification of Gastric Cancer Stem Cells Using the Cell S...
Table 1
Cell surface marker expression and tumorigenicity of human gastric cancer cell lines
AGS CD44+
https://clincancerres.aacrjournals.org/content/clincanres/early/2017/01/11/1078-0432.CCR-15-2157.full.pdf
We then analyzed the expression of seven additional cell surface markers as putative markers of gastric CSCs, i.e., CD10, CD49f, and CD166 described in CSCs of other organs (29–31), CD73, CD90, and CD105 as the main markers of mesenchymal stem cells (27, 32), as well as ALDH activity in cells from five PDXs (freshly collected at P2–P4) and five gastric carcinoma cell lines (seven intestinal type: GC04, GC10, GC35, GC44, MKN74, MKN7, NCI87; and three diffuse type: GC06, AGS, MKN45; Fig. 3A).
Figure 3. Expression of putative markers of CSCs and tumorigenic properties of corresponding cell subpopulations of PDXs and gastric carcinoma (GC) cell lines. A, the percentages of cells expressing EPCAM, CD24, CD133, CD44, CD10, CD49f, CD73, CD166, CD90, CD105, and ALDH activity were determined by flow cytometry analyses on 7-AAD cells from 5 PDXs after P2–P4 (PTs: GC04, GC06, GC10, GC35, and GC44) and 5 gastric carcinoma cell lines (CLs: AGS, MKN28, MKN45, MKN74, and NCI87) of diffuse and intestinal types.
CLs – diffuse
PTs – diffuse
Hello José,
You can check this article:
Article Identification of Stem-like Cells and Clinical Significance ...
Best regards
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