I am trying to print the negative of bone trabeculae then fill with hydroxyapatite to create a scaffold to seed cells on but haven't found a good HA slurry to use.
Lisa, Eoin Cunningham could maybe give advice on this: [email protected]
Filing a negative mold with a ceramic slurry it is a difficult task to optimise specially if you are fabricating a small scale construct. I suggest you to use polymer sponge method to make a scaffold resembling tubercular bone structure.
It is a difficult task to optimise a slurry to fill a negative mold specially if you have micron sized features on the final product. I recommend you to use polymer sponge method to fabricate a scaffold resembling trabecular bone structure.
Try to use BONIT matrix from DOT-coating.de, its porous material. But you can grinder it to a fine powder to which you could add an matrix builder? (Agarose, Gelatine??)
they have also some sponges with this stuff, which looks widely like trabeculare.
Normally “a good HA slurry” appears to be a multi-component suspension. Namely, besides fine HA powder and water, it contains various additives such as binders, surfactants, anti-foaming and anti-coagulation agents, etc. to simplify the production state. Usually, such HA slurries are used by scaffold producing companies and, therefore, both the precise compositions and preparation receipts are known but kept in a secret. Therefore, try to look in publications and/or Internet and, in the case of a failure, you will need to develop it by yourself.
After all the answers on the difficulty of filling a negative mold, we have started printing the scaffold and coated with collagen but I think adding HA to the collagen in some manner would be more beneficial. Any suggestions? Currently, the cells do not attach well to the collagen only scaffolds.
- Badges
- Science topic