Does anyone know a good c-myc Ab which works in blot on mouse liver extracts?

More Joaquim Javary's questions See All

What is the eucalypt surface in your country??

Dear colleague we are doing an update of the eucalypt surface all over the World. Could you please send me any avaliable information on this topic in your specific country or elsewhere? Please...

13 October 2021 9,034 9 View

Is it possible to easily aggregate relationships in NVIVO in a non-manual way?

Is there a way to aggregate an existing relationship between two sub-nodes to higher level nodes? For example, let us say you have "code 1" with an underlying "sub-code 1" and "code 2" with an...

07 March 2021 4,260 3 View

SDD disk applied to CCSD(T) calculations and ab initio?

Dear all, Recently the technology in SDD disk have been developed by increasing the read-write velocities and the "lifetime" of writes such hundreds of TB. Even so, the available memory is very...

06 April 2018 7,050 8 View

Anyone interested to get data from the Aliens & Flames project?

This is a rare opportunity to investigate the fire-invasion continuum by observing the conditions before, during and after fire. Likewise, these experiments may provide original data for other...

26 March 2018 4,372 4 View

Is there a tool to retrieve Halstead metrics from a basic PhP file?

Tools such as PhP metrics are limited to Object Oriented code and dependent on fixed structures. I'm searching for tools to analyse students non OO code. I've already tested: "Sonarqube",...

10 January 2018 8,298 2 View

Any practical reason for the constant denominator in Halstead's Difficulty measurement?

Difficulty = (n1/2) * (N1/n2). Is there any reason (other than have a smaller number) for dividing n1 by 2? Adicionaly, what are the main metrics based on Halstead's?

11 December 2017 9,341 1 View

How to control the increase of cost and time in construction projects?

Despite the importance to society, construction industry projects suffer frequent delays and cost increases (OLIVEIRI et al., 2016). Construction works have a huge waste and a low efficiency...

25 September 2017 1,987 10 View

Apriori measurements: support, confidence etc. Are the equations according to the R package 'rRules'?

Hello everyone.Regarding the R package arules. I've realized that (at least) two of the most common measurements for APRIORI are actually using intersection instead of union.Support, supp(A ∪ B...

16 September 2017 5,753 2 View

Which echocardiography markers have you used to asses left ventricular diastolic function?

left atrium volume? tissue doppler myocardial velocities? pulmonary venous flow? ratio E/e LV ?

27 June 2017 6,557 22 View

Does anybody have antibodies for any fructans enzymes?

For Immunolocalization

01 September 2016 4,586 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View