SDD disk applied to CCSD(T) calculations and ab initio?

Dear all, Recently the technology in SDD disk have been developed by increasing the read-write velocities and the "lifetime" of writes such hundreds of TB. Even so, the available memory is very...

06 April 2018 6,839 8 View

Apriori measurements: support, confidence etc. Are the equations according to the R package 'rRules'?

16 September 2017 5,650 2 View

Does anybody have antibodies for any fructans enzymes?

For Immunolocalization

01 September 2016 4,461 1 View

Which is the form of the initial NIR spectra Matrix for PLS regression?

Dear all, I am studying the calibration with PLS methods and, a point that I could not find, is the form that the initial matrix of the spectra is build. Which parameters are used? Is...

07 May 2016 8,788 3 View

CCSD(T) too much time, any help?

Dear, Actually I am performing a sp CCSD(T)/aug-cc-pvQz (in gaussian09) with around 720 basis functions and 1100 primitive gaussian of size (the compound has pseudo-potential core for a metal) on...

06 December 2015 4,833 9 View

Why B3LYP was not re-optimized?

When I was studying adiabatic methods (hybrids) the first to apply three parameters to correlation integral was Perdew, giving the B3PW91. Later, it was suggested to apply full correlation method...

23 March 2015 597 5 View

Problems with CCSD(T) variables?

Hello, Recently I start running ccsd(t) calculations in g09 but the output tells that a variables are required for run the job. I am gamess user it don't give this problem. I try searching the...

16 October 2014 3,401 2 View

Can anybody help me to implement GPU in CUDA?

Hello, Recently I have installed the CUDA 6.5 for window. On the other hand I have gamess.13-64.pgi.linux.blas working on a i7 4cores and I have a NVidia GT630 (2Gb).My intention was to include...

26 September 2014 9,009 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View