I am interested in doing two color PALM microscopy using mEOS2 + DRONPA.
You could find these two plasmids in Addgene.
https://www.addgene.org/54682/
https://www.addgene.org/54662/
I would like to slice frozen brain cortex from mice into two hemispheres in order to use them for two separate isolation methods. Any idea how this could be done without thawing the tissue. The...
10 November 2017 3,302 4 View
I am interested in performing Ubiquitination analysis from whole lysates, however most protocols enriching for Ubiquitinated peptides using the KGG motif (enriched using IP for KGG peptides) has...
03 April 2016 4,259 3 View
I am interested in a secreted protein and I express it in HEK cells. I see a band at a lower molecular weight (around 60 kDa) in the cell lysate, but a band at a higher molecular weight in the...
05 June 2014 6,149 1 View
I am interested in looking at proteome of single cells. Now I did a literature survey to find if anything of that sort has been done, but it doesnt seem so. I can think of some reasons, of course...
10 November 2013 7,392 49 View
I am essentially trying to separate native complexes using BNPAGE. I am trying to use precast tris-acetate gel 3-8% from Life Technologies. I was wondering if it's a good idea to run the samples...
09 October 2013 4,153 5 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
EDX analisys
31 July 2024 5,947 2 View