I am interested in doing two color PALM microscopy using mEOS2 + DRONPA.
You could find these two plasmids in Addgene.
https://www.addgene.org/54682/
https://www.addgene.org/54662/
I would like to slice frozen brain cortex from mice into two hemispheres in order to use them for two separate isolation methods. Any idea how this could be done without thawing the tissue. The...
10 November 2017 3,224 4 View
I am interested in performing Ubiquitination analysis from whole lysates, however most protocols enriching for Ubiquitinated peptides using the KGG motif (enriched using IP for KGG peptides) has...
03 April 2016 4,184 3 View
I am interested in a secreted protein and I express it in HEK cells. I see a band at a lower molecular weight (around 60 kDa) in the cell lysate, but a band at a higher molecular weight in the...
05 June 2014 6,082 1 View
I am interested in looking at proteome of single cells. Now I did a literature survey to find if anything of that sort has been done, but it doesnt seem so. I can think of some reasons, of course...
10 November 2013 7,316 49 View
I am essentially trying to separate native complexes using BNPAGE. I am trying to use precast tris-acetate gel 3-8% from Life Technologies. I was wondering if it's a good idea to run the samples...
09 October 2013 4,073 5 View
Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...
03 March 2021 6,193 1 View
Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru
02 March 2021 1,787 2 View
I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...
02 March 2021 6,270 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...
01 March 2021 3,052 3 View
During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...
01 March 2021 6,729 2 View
I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...
28 February 2021 6,477 1 View
Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...
28 February 2021 4,237 1 View
Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...
26 February 2021 8,399 5 View
Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...
25 February 2021 1,675 3 View
