Nikhil Pandya

6 Questions 13 Answers 0 Followers

Questions related from Nikhil Pandya

Cutting Frozen mouse Brain tissue without thawing?

I would like to slice frozen brain cortex from mice into two hemispheres in order to use them for two separate isolation methods. Any idea how this could be done without thawing the tissue. The...

11 November 2017 3,283 4 View

Scale down of Ubiquitination assay using PTMScan?

I am interested in performing Ubiquitination analysis from whole lysates, however most protocols enriching for Ubiquitinated peptides using the KGG motif (enriched using IP for KGG peptides) has...

04 April 2016 4,239 3 View

Does anyone know a company that sells these constructs in a destination vector format (thereby easing the cloning process)?

I am interested in doing two color PALM microscopy using mEOS2 + DRONPA.

08 August 2014 5,404 1 View

SDS PAGE Western Blot on cell culture medium?

I am interested in a secreted protein and I express it in HEK cells. I see a band at a lower molecular weight (around 60 kDa) in the cell lysate, but a band at a higher molecular weight in the...

06 June 2014 6,130 1 View

Single cell Proteomics using MS- How to do this?

I am interested in looking at proteome of single cells. Now I did a literature survey to find if anything of that sort has been done, but it doesnt seem so. I can think of some reasons, of course...

11 November 2013 7,368 49 View

Blue native gel electrophoresis using tris-acetate gels?

I am essentially trying to separate native complexes using BNPAGE. I am trying to use precast tris-acetate gel 3-8% from Life Technologies. I was wondering if it's a good idea to run the samples...

10 October 2013 4,131 5 View