Cecile Bienboire-Frosini

10 Questions 31 Answers 0 Followers

Questions related from Cecile Bienboire-Frosini

Does anyone know a commercial provider for a chitinase assay kit?

We would like to assess the chitinase activity in fish mucus sample, and have started to work with a chitinase assay kit from Sigma. Unfortunately, this one is out of stock since several...

27 May 2020 8,542 3 View

CSF puncture in rabbits?

Has anybody already perfomed CSF punctures in rabbits via the cisterna magna and could give me technical advice about it? How much CSF can be collected by this way?

01 June 2018 2,941 3 View

CSF puncture in rabbits?

Has anybody already perfomed CSF punctures in rabbits via the cisterna magna and could give me technical advice about it? How much CSF can be collected by this way?

17 April 2018 9,114 0 View

cDNA sequencing directly from purified PCR products or after cloning?

Could someone tell me the pros and cons of each, regarding the main downstream applications? Thank you!

18 September 2017 3,591 10 View

Does the use of RNAse inhibitor is mandatory in Reverse-Transcription experiments?

I'm using the Sensiscript Reverse Transcription kit from Qiagen which recommends that but I've also been told that it's not necessary as long as I work in a RNAse free environment thanks to...

20 April 2017 1,116 4 View

Can someone advise me about the choice of a Reverse Transcription kit to synthetize cDNA to get the full CDS of a transcript?

My transcript is not very long ( about 500 bp) but my first attempt to synthetize cDNA wiht total extracted RNA from biopsies failed to result in full CDS cDNA that I would need to perform...

17 September 2015 7,114 3 View

Has anyone already used the estradiol ELISA kit for bovine samples from ABNOVA (ref. KA2276)?

Surprisingly the provider (Abnova) does not recommend an extraction of estradiol from bovine sera samples before the assay, unlike protocols from other estradiol ELISA kit, and the sensitivity...

29 January 2015 5,908 2 View

Does anyone have experience with Fecal Glucorticoid Metabolites extraction and measurement from parrot (or at least bird) feces?

I would appreciate details regarding the extraction procedure such as the drying method (if so), the extraction buffer (methanol, ethanol...), and the assay method in EIA. Thanks.

09 January 2015 1,752 1 View

Does anyone know a device to collect urine (naturally given out) from cows or have experience in performing this?

We would need a device with a container large enough to collect the whole urine and tough enough to withstand the flow strength... and preferably commercially available. Thanks

02 October 2014 8,243 10 View

Does anyone know a commercially available ELISA kit able to measure prolactin in bovine plasma samples?

Prolactin being a protein hormone, it is species-specific and I could only find prolactin ELISA kit in dogs (and humans of course).

17 June 2014 7,065 4 View