I am looking for a coenzyme Q10 extraction protocol for fibroblasts, in order to be measured by HPLC. Most articles are very vague explaining their procedure.
I’ve found a very well described protocol for extraction from Takada and its collaborators this protocol they used it for extracting CoQ10 from hepatic cell, however I’ve found that this same protocol was used by Compagnoni and its collaborators for fibroblast, also they describe widely their detection protocol using an HPLC.
Here are the references:
1. Takada, M., Ikenoya, S., Yuzuriha, T., & Katayama, K. (1984). [17] Simultaneous determination of reduced and oxidized ubiquinones. Oxygen Radicals in Biological Systems, 147–155. doi:10.1016/s0076-6879(84)05020-5
2. Monzio Compagnoni, G., Kleiner, G., Bordoni, A., Fortunato, F., Ronchi, D., Salani, S., … Di Fonzo, A. (2018). Mitochondrial dysfunction in fibroblasts of Multiple System Atrophy. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1864(12), 3588–3597.doi:10.1016/j.bbadis.2018.09.018