Test to assess the adequacy of a sample size for statistical analysis?

I would like to know if there is an existing statistical test, like for instance the Kaiser-Meyer-Olkin test for sampling adequacy used for the PCA, to test if a sample size is numerically big...

20 November 2020 3,077 5 View

Agricultural Sensors: What detection ranges should they have for GHGs and ammonia?

Dear all, If you want to estimate ammonia, nitrous oxide, methane and carbon dioxide emission from: - arable soil - slurry storage - livestock barns which range of detection should have the...

19 January 2020 2,378 3 View

Where can I found the molar absorptivity or molar extinction coefficient (epsilon) of the Lumogen F Orange Dye 240 and Lumogen F Red Dye 305?

I am doing some simulations with the dyes dispersed in a PMMA and I want to test the device with different dye concentration (ppm). In order to do so, I need the molar absorptivity/molar...

17 July 2019 7,109 2 View

P-value estimation in correlation test?

Good afternoon, I have a trouble with the output obtained from a correlation test performed, using Spearman's correlation coefficient, with R Software. The output obtained is the...

10 June 2019 9,741 3 View

Funds for PhD?

Good morning, My name is Marcello Franchini and I'm a Research Fellow at the University of Udine (Italy). I obtained the possibility to perform a PhD abroad but, in order to perform it, I need to...

08 April 2019 4,895 3 View

How to find and interpret the Conserved and variables regions in the phylogenetic analyses?

Hello Every one! i am working on diversity, Genetic Variability and population structure of a single species. I did phytolgenetic analyses. Now i am in final stage of interpretation but stuck...

12 February 2019 7,270 3 View

There is something outside of language or everything is mediated by language?

Recently I interviewed Professor Hans Kellner Dodds on the relationship between experience and language. The argument of the teacher, based on readings of Auerbach, Jakobson and Barthes, is that...

27 October 2016 7,231 30 View

Is there anybody working on the parameterization of the relationship between attached and free-living bacteria in ocean biogeochemical models?

15 June 2015 6,299 3 View

What is the best way to induce drought stress in wheat seedlings?

I need to grow 3-d old seedlings of durum wheat under drought condition and see the effect on cell wall modifiing enzymes at root level. Do you suggest use of PEG (4000 - 6000 or 8000)?

15 May 2014 6,399 4 View

Definition of 'interactive sound environment'

hi, I am looking for a definition of 'interactive sound environment'. With this term I refer to any system that uses the user input to affect the real time generated sound, such as in a...

01 January 1970 1,929 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View