Does anyone have transposons that would share that can be used for mutagenesis of Bacillus species?

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Does anyone know a source for theta-replicating Staphylococcus plasmids that are compatible?

I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...

23 July 2024 6,968 0 View

How to maintain intestinal tissue resident cells (TRM) in culture?

I am attempting to culture tissue resident T cells (TRM) derived from fresh intestinal tissue for up to six days. I am finding that the viability of the T cells is low at D0, and a very small...

08 July 2024 4,887 2 View

Help with in vitro testing of candida biofilm susceptibility?

Hi all, Would anyone have any suggestions for articles and protocols for performing microdilution tests on candida biofilm? Do you recommend diluting the drug in the medium and adding it to the...

19 June 2024 4,420 3 View

How to reconstitute ATCC of B.ovatus?

I am trying to reconstitute the bacterium B.ovatus ATCC. it was first placed in a THIO broth and despite this we observed no turbidity. This was done 24h after the stick containing B.ovatus ATCC...

12 June 2024 1,644 3 View

Someone can help me with error prone PCR?

How can you regulate the number of random inserted mutations in error prone PCR?

14 May 2024 673 2 View

How do I mention translation in an IPA research project?

Good evening, My IPA (Interpretative Phenomenological Analysis) research project involves conducting interviews in a different country and in a different language. Considering that I will need to...

05 May 2024 6,147 2 View

False Discovery Rate -- how many p values do you correct?

I am using this code (below) to use FDR to correct p values because I ran three exploratory growth curve models. How many p values do you need to correct? Do I just correct all of the p values for...

02 May 2024 4,028 4 View

Help to detect aggrecan in WB, anyone has detected the 250KDa band in mouse brain homogenates?

We are interested in detecting aggrecan (250 KDa) by WB. No luck so far, are there any recommendations, such as running buffer, sample loading buffer? Thank you in advance!

05 April 2024 6,269 4 View

How design Specific Primers for Reverse Transcription?

Hi :) How should I design primers for (-)ssRNA samples from Influenza A and hRSV viruses? I need to select the protein, locate the gene sequence, and then use it to design primers. Should I...

04 April 2024 4,896 1 View

Can I use feces preserved in formaldehyde to perform the McMaster technique?

Can I use feces preserved in formaldehyde to perform the McMaster technique for Haemonchus research?

20 March 2024 1,339 0 View

What should I do in this situation ?

I'm an undergraduate university student conducting research on the effects of internet use on depression and anxiety among undergraduate Ghanaian university students: Mediation role of Internet...

31 July 2024 2,979 3 View

In teaching with Evidence-Based Practices for students with ASD, what are some that can benefit from the use of AI?

I am a student in a master's program for Special Education and I am curious to see what Evidence-Based Practices that are used in teaching individuals with Autism Spectrum Disorder benefit from...

30 July 2024 5,611 1 View

Methods of teaching economics?

Answer should be based on institutional level

29 July 2024 1,506 4 View

Given currenct advances in the AI and Smart Computation, what are the prospects of having truly Humanoid Computing Devices in support of Education?

Today, many priorities for improvements to teaching and learning are unmet. Educators seek technology-enhanced approaches addressing these priorities that would be safe, effective, and scalable....

26 July 2024 8,544 4 View

What is the most appropriate pedagogical approach that suits or is best for teaching Elementary school classes?

Teaching is expected to bring changes in behavior and any pedagogical challenges faced at high schools is as a result of foundation which is the elementary school. So, which teaching strategy or...

21 July 2024 2,815 5 View

I would to know what are 10 different AI tools used in language research?

I would like to wirte an article on the use of AI tools in teaching speaking skills. What are the relevant AI tools that I can use to teach and guide students to use them? Thank you

13 July 2024 2,301 4 View

Does creative teaching influences positively in students' learning?

Creative teaching can lead to a wide variety of teaching styles.

12 July 2024 8,627 4 View

How well are first year teachers prepared to teach Early Literacy skills?

research on teacher preparation programs and readiness to teach early literacy skills

10 July 2024 2,177 5 View

Can anyone give me the name of Universities which accept PHD after undergraduate degree ?

Any Universities which accept students to PHD program after a undergraduate degree.

06 July 2024 3,903 2 View

How can technology empower EFL teaching?

Scholars from multiple disciplines stressed the importance of AI Technology in ESL teaching from a theoretical perspective. Are there teaching designs that combine theoretical discussions and...

01 July 2024 999 7 View