Claudia Gentry-Weeks

7 Questions 6 Answers 0 Followers

Questions related from Claudia Gentry-Weeks

Does anyone know of a good place to (low cost, reliable) get phage DNA sequenced?

I teach a course 'Phage Discovery and Genetics' to 3rd year undergraduate students. I have 6 phage DNA samples that I need to have sequenced as soon as possible. I need someplace that is...

27 April 2025 2,271 3 View

Does anyone know a source for theta-replicating Staphylococcus plasmids that are compatible?

I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...

24 July 2024 7,243 0 View

Methods to inhibit recovery of Bacillus species from soil?

My class is isolating antibiotic-producing bacteria from soil. The majority of the isolates are Bacillus species since we use nonselective media (grown on trypticase soy agar, LB, or potato...

11 June 2021 1,329 3 View

Does anyone know how cosmid pCP13 was constructed? Does anyone know the source of the Kan resistance gene in this cosmid?

I need the DNA sequence pCP13 cosmid vector. I know that pCP13 was derived from pLAFR1 and have the DNA sequence for pLAFR1 but I don't know the sequence of the kanamycin resistance gene (or where...

30 July 2019 6,257 2 View

Does anyone have Pseudomonas phage phiKZ that you are willing to share? Will pay shipping costs. Thanks!

I am cloning endolysins and need phage phiKZ. I will gladly pay shipping costs. Please email me at [email protected] if you have this phage and will share it. Thank you!

06 July 2017 2,433 0 View

Does anyone have transposons that would share that can be used for mutagenesis of Bacillus species?

Hello, I am looking for some transposons (mariner based or otherwise) that I can use in my undergraduate teaching lab for mutagenesis of Bacillus species that have been isolated from soil. If...

19 April 2017 3,606 2 View

Where to obtain plasmids - pAW016, pAW068, and pIC333?

Hello, I am trying to find the plasmids pAW016, pAW068, and pIC333 for transposon mutagenesis of Paenibacillus. I have tried many searching many repositories and have reached out to the authors of...

01 January 1970 2,486 2 View