Claudia Gentry-Weeks

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Questions related from Claudia Gentry-Weeks

Does anyone know a source for theta-replicating Staphylococcus plasmids that are compatible?

I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...

24 July 2024 6,938 0 View

Methods to inhibit recovery of Bacillus species from soil?

My class is isolating antibiotic-producing bacteria from soil. The majority of the isolates are Bacillus species since we use nonselective media (grown on trypticase soy agar, LB, or potato...

11 June 2021 1,273 3 View

Does anyone know how cosmid pCP13 was constructed? Does anyone know the source of the Kan resistance gene in this cosmid?

I need the DNA sequence pCP13 cosmid vector. I know that pCP13 was derived from pLAFR1 and have the DNA sequence for pLAFR1 but I don't know the sequence of the kanamycin resistance gene (or where...

30 July 2019 6,177 2 View

Where to obtain plasmids - pAW016, pAW068, and pIC333?

Hello, I am trying to find the plasmids pAW016, pAW068, and pIC333 for transposon mutagenesis of Paenibacillus. I have tried many searching many repositories and have reached out to the authors of...

01 January 1970 2,428 2 View