Hallo,

I have been trying to isolate Monocytes from PBMC (obtained though Density gradient from a buffy coat). to get non labeld monocytes, I used the depletion program according to the Classical Monocyte isolation Kit Data sheet. after the separation process I did a FACS analysis on a sample of the cells and the purity of the sample was very bad. One can say that the isolation didn’t work at all… does anyone have experience with this kit? Or with the isolation of Monocytes from PBMCs?

thanks in advance :)