I would like to FACS sort (after labeling PBMC sample with specific cell surface antibody cocktails) the PBMC cell types (like CD4+T cells, CD8+ T cells, B cells, DCs, NK cells etc) and culture them for my further experiments. Can you people share what are the growth factors/cytokines you add to culture/propagate the cell types given above? If you are doing the similar kind of work or regularly culture any of these cell types after FACS sorting, please let me know. I am digging up existing literature, but it would be of great help if you kindly share your well-worked culture conditions. Any suggestion welcome.
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