Hello, i had worked on this years back, please use the link below and read my publication on this which outlines the complete methodology of isolating vibrio cholerae from water:

http://www.ccsenet.org/journal/index.php/gjhs/article/view/10707/8712

Hi Terence, I will check this out. Thank you!

Please contact Mr. Sylvain Traoré.

we are looking at antibiotic rsistance of vibrio isolates and we have found MDR Vibrio cholerae isolates. another hypothesis i am trying to prove is recent mass oral cholera vaccine is inducing genetic mutations in v.cholerae. you can immunize and try to follow immunized persons excreting altered vibrio

Hi April,

the CDC pretty well describes the standard procedures: http://www.cdc.gov/cholera/pdf/laboratory-methods-for-the-diagnosis-of-vibrio-cholerae-chapter-4.pdf

Hi April,

I think the suggestion of Benedikt Ley to refer to CDC procedures is very helpful. V. cholerae is not a very hazardous agent. See about at http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/vibrio-cholerae-eng.php. The site of Public Health Agency of Canada contains exhaustive and easy to check forms for the management of most pathogens in lab

Working with Vibrio cholerae requires Biosafety level 2 precautions, which is a bit more strict than working with general, non-infectious microbial agents. See for example:

http://www.safety.duke.edu/SafetyManuals/Lab/Section1BiologicalSafety/Chapt%208%20BIOSAFETY%20LEVELS.pdf

Having worked with Vc myself, one thing I remember is that growing the bacterium at lower temperatures (say, 25 degrees C) makes the bacterium much more virulent than growing at 37 degrees.